Getting the dose right with 5-Amino-1MQ is one of the most common questions researchers face when working with this NNMT inhibitor. Unlike reconstitutable peptides, 5-Amino-1MQ is a small molecule typically supplied as a powder or in capsule form — but the dosage math still matters for reproducible results. For peptides that do require reconstitution, our free peptide dosage calculator handles the conversions instantly.
Preclinical Dosage Parameters
The most-cited preclinical study (Neelakantan et al., 2019) used 5-Amino-1MQ at 50 mg/kg/day via IP injection in a diet-induced obesity mouse model over 11 days. At this dose, treated mice showed significantly reduced body weight gain, decreased fat mass, and improved metabolic markers compared to controls.
It is important to note that direct mg/kg translation from rodent to human dosing is not straightforward. Allometric scaling and species-specific metabolic differences mean that human-equivalent doses are substantially lower on a per-kg basis.
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Try the Calculator →Common Research Protocols
In non-clinical research settings, 5-Amino-1MQ is most commonly referenced at doses in the range of 50–150 mg per day orally. Protocol length in anecdotal research logs typically spans 4–8 weeks, though no standardized human protocol exists in published literature.
Key protocol considerations include:
- Administration route: Oral (capsule or dissolved in water) — no reconstitution needed
- Timing: Typically taken once daily, morning dosing preferred in research notes
- Cycle length: 4–8 weeks in most documented protocols, with breaks between cycles
- Stacking: Sometimes combined with NAD+ precursors (NMN or NR) in metabolic research protocols
Dose-Response Observations
Preclinical data suggests a clear dose-response curve for fat mass reduction. The Neelakantan study demonstrated that higher concentrations of 5-Amino-1MQ produced greater inhibition of NNMT enzymatic activity in vitro, with an IC50 of approximately 1.2 μM. This dose-dependent inhibition translated to proportional changes in adipocyte differentiation markers.
Solubility & Preparation Notes
5-Amino-1MQ is water-soluble at research-relevant concentrations. For in vitro work, it dissolves readily in PBS or culture media at concentrations up to 10 mM. For oral research preparations, it can be dissolved in plain water without the need for co-solvents like DMSO or PEG-400.
This water solubility distinguishes it from most peptide research compounds, which require careful reconstitution with bacteriostatic water and precise syringe measurements.
Storage & Stability
Lyophilized 5-Amino-1MQ powder should be stored at -20°C for long-term stability. Once dissolved, solutions should be kept refrigerated (2–8°C) and used within 7–14 days. Avoid repeated freeze-thaw cycles, as stability data beyond 3 cycles has not been published.
How This Differs from Peptide Dosing
Unlike BPC-157, TB-500, or GHK-Cu — which require reconstitution with bacteriostatic water and precise syringe volume calculations — 5-Amino-1MQ dosing is straightforward weight-based measurement. However, researchers working with mixed protocols that include both 5-Amino-1MQ and injectable peptides should use our peptide reconstitution calculator for the injectable components to ensure accuracy.